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Phospho-YAP1 (Ser127) Recombinant Rabbit Monoclonal Antibody

Catalog Number: orb2563466

DispatchUsually dispatched within 5-10 working days
$ 270.00
Catalog Numberorb2563466
CategoryAntibodies
DescriptionPhospho-YAP1 (Ser127) Recombinant Rabbit Monoclonal Antibody
TargetYAP1
ClonalityRecombinant
Species/HostRabbit
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman
Predicted ReactivityMouse, Rat
Form/AppearanceLiquid
Concentration1mg/ml
Buffer/Preservatives0.01M TBS(pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenKLH conjugated Synthesised phosphopeptide derived from human YAP around the phosphorylation site of Ser127 AH(p-S)SP
UniProt IDP46937
MW55 kDa
Tested applicationsFC, WB
Dilution rangeWB=1:500-3000, Flow-Cyt=2ug/Test
Antibody TypeRecombinant Antibody
Clone Number10C1
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesYAP1(phospho Ser127); YAP1(phospho S127); 65 kDa Y
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NoteFor research use only
Phospho-YAP1 (Ser127) Recombinant Rabbit Monoclonal Antibody

Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-Phospho-YAP1 (Ser127) antibody (orb2563466), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Phospho-YAP1 (Ser127) Recombinant Rabbit Monoclonal Antibody

Sample: Lane 11: Human HeLa cell lysates, Lane 12: Human HeLa treated with 100 ng/ml Calyculin A for 30 min cell, Primary: Anti-Phospho-YAP1 (Ser127) (orb2563466) at 1/3000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kDa, Observed band size: 65 kDa.

  • Phospho-YAP1 (Ser127) Recombinant Rabbit Monoclonal Antibody [orb559171]

    IF,  IHC-Fr,  IHC-P,  WB

    Mouse

    Human, Mouse, Rat

    Rabbit

    Recombinant

    Unconjugated

    100 μl, 50 μl, 25 μl