Cart summary

You have no items in your shopping cart.

Phospho-YAP1 (Ser127) Recombinant Rabbit Monoclonal Antibody

Catalog Number: orb559171

DispatchUsually dispatched within 5-10 working days
$ 200.00
Catalog Numberorb559171
CategoryAntibodies
DescriptionPhospho-YAP1 (Ser127) Recombinant Rabbit Monoclonal Antibody
TargetYAP1
ClonalityRecombinant
Species/HostRabbit
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Predicted ReactivityMouse
Form/AppearanceLiquid
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenKLH conjugated Synthesised phosphopeptide derived from human YAP around the phosphorylation site of Ser127 AH(p-S)SP
UniProt IDP46937
MW55 kDa
Tested applicationsIF, IHC-Fr, IHC-P, WB
Dilution rangeWB=1:500-5000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200
Antibody TypeRecombinant Antibody
Clone Number1H12
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesphospho-YAP1(S127); YAP1(S127); YAP1(phospho Ser12
Read more...
NoteFor research use only
Phospho-YAP1 (Ser127) Recombinant Rabbit Monoclonal Antibody

Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-Phospho-YAP1 (Ser127) antibody (orb559171), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Phospho-YAP1 (Ser127) Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Phospho-YAP1 (Ser127) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Phospho-YAP1 (Ser127) Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Phospho-YAP1 (Ser127) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Phospho-YAP1 (Ser127) Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human Duodenum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Phospho-YAP1 (Ser127) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Phospho-YAP1 (Ser127) Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human Fundus, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Phospho-YAP1 (Ser127) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Phospho-YAP1 (Ser127) Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Phospho-YAP1 (Ser127) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Phospho-YAP1 (Ser127) Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Rat Stomach, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Phospho-YAP1 (Ser127) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

  • Phospho-YAP1 (Ser127) Recombinant Rabbit Monoclonal Antibody [orb2563466]

    FC,  WB

    Mouse, Rat

    Human

    Rabbit

    Recombinant

    Unconjugated

    50 μl, 100 μl