Phospho-Tau (Thr181)

• Catalog Number: orb1294393
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Tau (Phospho Thr181)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide corresponding to residues surrounding Thr181 of human Tau protein
• Dilution range: Western Blot 1:500-1:1500 Immunofluorescence 1:200–1:400 Immunohistochemistry (Paraffin) 1:100
• Conjugation: Unconjugated
• Target: Phospho-Tau (Thr181)
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-Phospho-Tau (Thr181) antibody at 1/1000 dilution, Lysates/proteins at 55 μg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294393 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human tissue labeling phosphor-Tau (Thr181) with orb1294393 at 1/100.

Specificity and sensitivity of Phospho-Tau (Thr181) Antibody (orb1294393).

Immunohistochemical of frozen sections. Phospho-Tau (Thr181) antibody (orb1294393): 1-200; Red: Tuj1 antibody; Anti-rabbit 488: 1-500.

Anti-Phospho-Tau (Thr181) antibody at 1/1000 dilution, Lysates/proteins at 55 μg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294393 overnight at 4°C.

Immunofluorescent analysis. Sample: cortical neurons; Phospho-Tau (Thr181) antibody (orb1294393): 1-200; Red: MAP2 antibody; ; Anti-rabbit 488: 1-500; Fixed: 4% paraformaldehyde at RT for 20 min.