Phospho-Tau Protein (Thr181) Rabbit Polyclonal Antibody

• Catalog Number: orb500682
• Category: Antibodies
• Description: Phospho-Tau Protein (Thr181) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Tau around the phosphorylation site of Thr181 PK(p-T)PP
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 52/79 kDa
• Target: MAPT
• UniProt ID: P10636
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Tau(phospho T181); p-Tau(phospho T181); Tau(phosph
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Tau Protein (Thr181)) Polyclonal Antibody, Unconjugated (orb500682) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-Tau Protein (Thr181) (orb500682) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kD, Observed band size: 52 kD.

Sample: K562 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-Tau Protein (Thr181) (orb500682) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kD, Observed band size: 52 kD.

Sample: Lane 1: Human Tau-F Protein at 500 ng, Primary: Rabbit Anti-phospho-Tau Protein Antibody at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45.8kD, Observed band size: kD.