Phospho-Stat5 (Tyr694) (G11) rabbit mAb

• Catalog Number: orb1946100
• Category: Antibodies
• Description: Phospho-Stat5 (Tyr694) (G11) rabbit mAb
• Clonality: Monoclonal
• Clone Number: Stat5Y694-G11
• Tested applications: FC, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgGk
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Tyr694 of human phospho Stat5
• Concentration: 0.5 mg/ml
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Conjugation: Unconjugated
• UniProt ID: P42229, P51692
• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Alternative names: Signal transducer and activator of transcription 5
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of K562 cell extract untreated or treated with imatinib using 0.01μg/mL Phospho-Stat5 (Tyr694) antibody Stat5Y694-G11. Cat. #orb1946100. Also shown is a control blot at the same exposure level and omitting only the primary antibody.

Peptide blocking flow cytometric analysis of 3T3 cells secondary antibody only negative control (light blue) or treated with imatinib (red) or pervanadate-treated (green) or imatinib and blocked with phospho-peptide (black) or pervanadate and blocked with phospho peptide (gold) or imatinib and blocked with non-phospho peptide (dark blue) or pervanadate and blocked with non-phospho peptide (purple) using Phospho-Stat5 (Tyr694) antibody Stat5Y694-G11 at 0.1 μg/mL. Cat. #orb1946100.

Flow cytometric analysis of 3T3 cells secondary antibody only negative control (blue) or untreated (grey) or treated with pervanadate (orange) using 0.1 μg/mL isotype control Cat. #orb1946241 or untreated (red) or pervanadate (green) using Phospho-Stat5 (Tyr694) antibody Stat5Y694-G11 at 0.1 μg/mL. Cat #orb1946100.

Western blot analysis of K562 cell extract untreated or treated with imatinib using 0.01 μg/mL Phospho-Stat5 (Tyr694) antibody Stat5Y694-G11 Cat. #orb1946100 or Company C antibody at 0.1 μg/mL (manufacturer's recommended concentration) developed using the same exposure.

Flow cytometric analysis of Ramos cells untreated (red) or treated with IFNa + IL-4 + Pervanadate (green) using Phospho-STAT5 (Tyr694) (G11) rabbit mAb STAT5Y694-G11 at 0.01 ug/mL #orb1946100, or concentration-matched rabbit (G9) mAb IgG Isotype Control #orb1946241 for cells untreated (gray) or treated with IFNa + IL-4 + pervanadate (blue).