Phospho-Stat5 (Tyr694) (B5) rabbit mAb Antibody

• Catalog Number: orb1946160
• Category: Antibodies
• Description: Phospho-Stat5 (Tyr694) (B5) rabbit mAb Antibody
• Clonality: Monoclonal
• Clone Number: Stat5Y694-B5
• Tested applications: FC
• Reactivity: Human, Mouse
• Isotype: Rabbit IgGk
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Tyr694 of human phospho Stat5
• Concentration: 0.5 mg/ml
• Dilution range: 1.00 - 0.001 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Conjugation: Unconjugated
• UniProt ID: P51692, P42229
• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Alternative names: Signal transducer and activator of transcription 5
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of Ramos cells untreated (red) or treated with IFNa + IL-4 + pervanadate (green) using Phospho-Stat5 (Tyr694) antibody at 0.05 ug/mL, Stat5Y694-B5 #orb1946160, or concentration-matched rabbit (G9) mAb IgG Isotype Control #orb1946241 for cells untreated (black) or treated with IFNa + IL-4 + pervanadate (blue).

Peptide blocking flow cytometric analysis of K562 cells secondary antibody only negative control (light blue) or treated with imatinib (red) or pervanadate-treated (green) or imatinib and blocked with phospho-peptide (black) or pervanadate and blocked with phospho peptide (gold) or imatinib and blocked with non-phospho peptide (dark blue) or pervanadate and blocked with non-phospho peptide (purple) using Phospho-Stat5 (Tyr694) antibody Stat5Y694-B5 at 0.1 μg/mL. Cat. #orb1946160.

Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 μg/mL of isotype control Cat. #orb1946241 (orange) or treated with imatinib (red) or with pervanadate (green) using Phospho-Stat5 (Tyr694) antibody Stat5Y694-B5 at 0.1 μg/mL. Cat #orb1946160.

Flow cytometric analysis of 3T3 cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using 0.1 ug/mL of Phospho-Stat5 (Tyr694) antibody Stat5Y694-B5 (Cat. #orb1946160) or Company C antibody at 0.4 ug/mL (manufacturer's recommended concentration).