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Catalog Number | orb1946160 |
---|---|
Category | Antibodies |
Description | Phospho-Stat5 (Tyr694) (B5) rabbit mAb |
Clonality | Monoclonal |
Clone Number | Stat5Y694-B5 |
Tested applications | FC |
Reactivity | Human, Mouse |
Isotype | Rabbit IgGk |
Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Tyr694 of human phospho Stat5 |
Concentration | 0.5 mg/ml |
Dilution range | 1.00 - 0.001 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information. |
Conjugation | Unconjugated |
UniProt ID | P42229, P51692 |
Storage | -20°C |
Buffer/Preservatives | 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin |
Alternative names | Signal transducer and activator of transcription 5 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Flow cytometric analysis of Ramos cells untreated (red) or treated with IFNa + IL-4 + pervanadate (green) using Phospho-Stat5 (Tyr694) antibody at 0.05 ug/mL, Stat5Y694-B5 #orb1946160, or concentration-matched rabbit (G9) mAb IgG Isotype Control #orb1946241 for cells untreated (black) or treated with IFNa + IL-4 + pervanadate (blue).
Peptide blocking flow cytometric analysis of K562 cells secondary antibody only negative control (light blue) or treated with imatinib (red) or pervanadate-treated (green) or imatinib and blocked with phospho-peptide (black) or pervanadate and blocked with phospho peptide (gold) or imatinib and blocked with non-phospho peptide (dark blue) or pervanadate and blocked with non-phospho peptide (purple) using Phospho-Stat5 (Tyr694) antibody Stat5Y694-B5 at 0.1 μg/mL. Cat. #orb1946160.
Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 μg/mL of isotype control Cat. #orb1946241 (orange) or treated with imatinib (red) or with pervanadate (green) using Phospho-Stat5 (Tyr694) antibody Stat5Y694-B5 at 0.1 μg/mL. Cat #orb1946160.
Flow cytometric analysis of 3T3 cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using 0.1 ug/mL of Phospho-Stat5 (Tyr694) antibody Stat5Y694-B5 (Cat. #orb1946160) or Company C antibody at 0.4 ug/mL (manufacturer's recommended concentration).