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Description
Research Area
Stem Cell & Developmental Biology
Images & Validation
−
| Tested Applications | ELISA, IF, IHC |
|---|---|
| Dilution Range | IHC-P: 1:50-1:100, IF: 1:100-1:200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Peptide sequence around phosphorylation site of serine 1177 (T-Q-S(p)-F-S) derived from Human eNOS. |
| Target | NOS3 |
| Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | liquid |
| Buffer/Preservatives | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−cNOS antibody; Constitutive NOS antibody; EC NOS antibody; EC-NOS antibody; ecNOS antibody; Endothelial nitric oxidase synthase antibody; Endothelial nitric oxide synthase antibody; Endothelial nitric oxide synthase 3 antibody; Endothelial NOS antibody; eNOS antibody; Nitric oxide synthase 3 (endothelial cell) antibody; Nitric oxide synthase 3 antibody; Nitric oxide synthase 3 endothelial cell antibody; Nitric oxide synthase endothelial antibody; Nitric oxide synthase; endothelial antibody; NOS 3 antibody; NOS III antibody; NOS type III antibody; NOS3 antibody; NOS3_HUMAN antibody; NOSIII antibody
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Quick Database Links
Gene Symbol
NOS3
UniProt
UniProt Details
− No UniProt data available
Protocol Information
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)




























