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| Catalog Number | orb6519 |
|---|---|
| Category | Antibodies |
| Description | Phospho-eNOS (Ser1177) Rabbit Polyclonal Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, FC, IF, IHC-Fr, IHC-P, WB |
| Predicted Reactivity | Bovine, Canine, Guinea pig, Mouse, Porcine, Rabbit, Rat, Sheep |
| Reactivity | Human |
| Isotype | IgG |
| Immunogen | KLH conjugated Synthesised phosphopeptide derived from mouse eNOS around the phosphorylation site of Ser1177 TQ(p-S)FS |
| Antibody Type | Primary Antibody |
| Concentration | 1mg/ml |
| Dilution range | WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2μg /Test, ELISA=1:2000-10000 |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | 133 kDa |
| Target | NOS3 |
| UniProt ID | P70313 |
| RRID | AB_10931821 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Alternative names | eNOS(phospho S1177); eNOS(phospho S1177); p-eNOS(p Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
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Hoetzenecker, K. et al. Secretome of apoptotic peripheral blood cells (APOSEC) attenuates microvascular obstruction in a porcine closed chest reperfused acute myocardial infarction model: role of platelet aggregation and vasodilation Basic Res Cardiol, 107, 292 (2012)
Applications
IHC
Reactivity
Porcine
Lan, Wenya et al. Human urine kininogenase attenuates balloon-induced intimal hyperplasia in rabbit carotid artery through transforming growth factor β1/Smad2/3 signaling pathway J Vasc Surg, 64, 1074-1083 (2016)
Applications
WB
Reactivity
Rabbit
Sample: Lane 1: Human HUVEC cell lysates, Lane 2: Human HUVEC treated with 100 µM Pervanadate (PV) for 10 minutes, Primary: Anti-Phospho-eNOS (Ser1177) (orb6519) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 133 kDa, Observed band size: 135 kDa.
The blue histogram is unstained cells (HepG 2 cells). The Orange histogram is cells stained with Rabbit IgG/FITC (orb525556), The green histogram is cells stained with Rabbit Anti-Phospho-eNOS (Ser1177)/FITC Conjugated antibody. Isotype control: Cell lines treated with Rabbit IgG/FITC (orb525556) instead of the primary antibody to confirm that primary antibody binding is specific. 2 µg in 100 µl 1X PBS containing 0.5% BSA.
