Phospho-eNOS (Ser1177) Rabbit Polyclonal Antibody

• Catalog Number: orb6519
• Category: Antibodies
• Description: Phospho-eNOS (Ser1177) Rabbit Polyclonal Antibody
• Target: NOS3
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from mouse eNOS around the phosphorylation site of Ser1177 TQ(p-S)FS
• UniProt ID: P70313
• RRID: AB_10931821
• MW: 133 kDa
• Tested applications: WB
• Dilution range: WB=1:500-1000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: eNOS(phospho S1177); eNOS(phospho S1177); p-eNOS(p
• Research Area: Cardiovascular Research, Hypoxia, Neuroscience, Ni
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Hoetzenecker, K. et al. Secretome of apoptotic peripheral blood cells (APOSEC) attenuates microvascular obstruction in a porcine closed chest reperfused acute myocardial infarction model: role of platelet aggregation and vasodilation Basic Res Cardiol, 107, 292 (2012)

PubMed: 22899170

Applications

IHC

Reactivity

Porcine

Lan, Wenya et al. Human urine kininogenase attenuates balloon-induced intimal hyperplasia in rabbit carotid artery through transforming growth factor β1/Smad2/3 signaling pathway J Vasc Surg, 64, 1074-1083 (2016)

PubMed: 26054589

Applications

WB

Reactivity

Rabbit

Sample: Lane 1: Human HUVEC cell lysates, Lane 2: Human HUVEC treated with 100 µM Pervanadate (PV) for 10 minutes, Primary: Anti-Phospho-eNOS (Ser1177) (orb6519) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 133 kDa, Observed band size: 135 kDa.

The blue histogram is unstained cells (HepG 2 cells). The Orange histogram is cells stained with Rabbit IgG/FITC (orb525556), The green histogram is cells stained with Rabbit Anti-Phospho-eNOS (Ser1177)/FITC Conjugated antibody. Isotype control: Cell lines treated with Rabbit IgG/FITC (orb525556) instead of the primary antibody to confirm that primary antibody binding is specific. 2 µg in 100 µl 1X PBS containing 0.5% BSA.