Phospho-NFkB p65 (Ser529) (H3) rabbit mAb Antibody

SKU: orb1946039

Description

The nuclear factor kB (NFkB)/Rel family of transcription factors play a pivotal role in inflammatory and immune responses (1,2). NF-kappa-B is present in almost all cell types and is involved in many biological processes including immunity, inflammation, cell growth and differentiation, apoptosis, and tumorigenesis. NFkB is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFkB1/p105, NFkB1/p50, REL and NFkB2/p52. The dimers bind at kB sites in the target gene DNA. Individual dimers have distinct preferences for different kB sites and can act as either transcriptional activators or repressors. NFkB Ser536 phosphorylation stimulates Lys310 acetylation and interaction of phospho NFkB with CBP. Acetylated/phospho NFkB induces enhanced transcriptional activity.

Images & Validation

• Tested Applications: FC
• Dilution Range: 1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG1k
• Clone No.: NFkBp65S529-H3
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser529 of human phospho-NFKB p65
• Purification: Protein A+G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Concentration: 0.5 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA, NFKB3

Flow cytometric analysis of HeLa cells secondary antibody only negative control (blue) or untreated (red) or treated with TNFα + calyculin A (green) using Phospho-NFkB p65 (Ser529) antibody NFkBp65S529-H3 at 0.01μg/mL. Cat. #orb1946039.

Peptide blocking flow cytometric analysis of HeLa cells secondary antibody only negative control (light blue) or untreated (red) or treated with TNFα + calyculin A (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-NFkB p65 (Ser529) antibody NFkBp65S529-H3 at 0.01μg/mL. Cat. #orb1946039.

Flow cytometric analysis of C2C12 cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using Phospho-NFkB p65 (Ser529) antibody NFkBp65S529-H3 at 1μg/mL. Cat. #orb1946039.

Quick Database Links

UniProt

Q04206