Phospho-NFkB p65 (Ser529) (A2) rabbit mAb Antibody

SKU: orb1946040

Description

The nuclear factor kB (NFkB)/Rel family of transcription factors play a pivotal role in inflammatory and immune responses (1,2). NF-kappa-B is present in almost all cell types and is involved in many biological processes including immunity, inflammation, cell growth and differentiation, apoptosis, and tumorigenesis. NFkB is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFkB1/p105, NFkB1/p50, REL and NFkB2/p52. The dimers bind at kB sites in the target gene DNA. Individual dimers have distinct preferences for different kB sites and can act as either transcriptional activators or repressors. NFkB Ser536 phosphorylation stimulates Lys310 acetylation and interaction of phospho NFkB with CBP. Acetylated/phospho NFkB induces enhanced transcriptional activity.

Research Area

Signal Transduction

Images & Validation

• Tested Applications: WB
• Dilution Range: 1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG1k
• Clone No.: NFkBp65S529-A2
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser529 of human phospho-NFKB p65
• Purification: Protein A+G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Concentration: 0.5 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA, NFKB3

Western blot analysis of NIH3T3 cell extract untreated or treated with TNFα and calyculin A using Phospho-NFkB p65 (Ser529) antibody NFkBS529-A2 at 0.05 μg/mL. Cat. #orb1946040.

Western blot analysis of NIH3T3 cell extract untreated or treated with TNFα + calyculin A using 0.05 μg/mL Phospho-NFkB p65 (Ser529) antibody NFkBS529-A2 or Company A antibody at 1:1000 dilution (manufacturer's recommended concentration) developed using the same exposure.

Quick Database Links

UniProt

Q04206