Phospho-Lck (Tyr505) (A3) rabbit mAb

• Catalog Number: orb1946120
• Category: Antibodies
• Description: Phospho-Lck (Tyr505) (A3) rabbit mAb
• Clonality: Monoclonal
• Clone Number: LckY505-A3
• Tested applications: FC, WB
• Reactivity: Human
• Isotype: Rabbit IgGk
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Tyr505 of human phospho Lck
• Concentration: 0.5 mg/ml
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Conjugation: Unconjugated
• UniProt ID: P06239
• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Alternative names: Tyrosine-protein kinase Lck, Leukocyte C-terminal
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Jurkat cell extract untreated or treated with 200nM calyculin A for 30min using Phospho-Lck (Tyr505) antibody LckY505-A3 at 0.1 μg/mL. Cat. #orb1946120.

Peptide blocking flow cytometric analysis of Daudi cells secondary antibody only negative control (light blue) or untreated (red) or treated with IFNα + IL-4 + pervanadate (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-Lck (Tyr505) antibody LckY505-A3 at 1 μg/mL. Cat. #orb1946120.

Flow cytometric analysis of Daudi cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNα + IL-4 + pervanadate (green) using Phospho-Lck (Tyr505) antibody LckY505-A3 at 1 μg/mL. Cat. #orb1946120.

Western blot analysis of Jurkat cell extract, untreated or treated with calyculin A using 0.1 μg/mL Phospho-Lck (Tyr505) antibody LckY505-A3 Cat. #orb1946120 or Company C antibody at 1 μg/mL (manufacturer's recommended concentration) developed using the same exposure.