Phospho-Lck (Tyr505) (A3) rabbit mAb APC conjugate Antibody

SKU: orb1946117

Description

Lck is a member of the Src family of non-receptor tyrosine kinases and plays a major role in T cell activation. Lck activates many downstream signaling pathways including Akt/mTOR, SAPK/JNK, PLCg1, and RAS/MAPK. Phosphorylation of Lck at Tyr394 in the catalytic domain at the ATP-binding site stabilizes the open and active form, while phosphorylation at Tyr505 in the C-terminal domain promotes the closed, inactive conformation. Multiple small-molecule drugs used to treat leukemia have been shown to target inhibition of Lck, including imatinib and dasatinib. Lck is thus a promising target for suppressing T-cell responses for the treatment of inflammatory diseases or after organ transplantation.

Images & Validation

• Tested Applications: FC
• Dilution range: For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG1k
• Clone No.: LckY505-A3
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Tyr505 of human phospho Lck
• Purification: Protein A+G

Storage & Handling

• Storage: 2-8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: 1X PBS, 0.09% NaN3, 0.2% rAlbumin
• Disclaimer: For research use only

Alternative Names

Tyrosine-protein kinase Lck, Leukocyte C-terminal Src kinase, LSK, Protein YT16, T cell-specific protein-tyrosine kinase

Flow cytometric analysis of Daudi cells unstained and untreated as negative control (blue) or untreated (red) or treated with IFNa plus IL4 (green) using Phospho-LCK (Y505) antibody LCKY505-A3 PE conjugate. Cat. #orb1946117.

Quick Database Links

UniProt

P06239