Phospho-Histone H3 (Ser28) (D6) rabbit mAb

• Catalog Number: orb1946316
• Category: Antibodies
• Description: Phospho-Histone H3 (Ser28) (D6) rabbit mAb
• Clonality: Monoclonal
• Isotype: Rabbit IgGk
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Purification: Protein A+G
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser28 of human phospho Histone H3
• UniProt ID: Q16695
• Tested applications: FC, WB
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Cross Reactivity: Predicted to work with mouse, rat and other homologues.
• Clone Number: HisH3S28-D6
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Histone H3.1t, H3t, H3FT, HIST3H3
• Note: For research use only

Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 μg/mL of isotype control Cat. #orb1946241 (orange) or untreated (red) or treated with UV and PMA (green) using Phospho-Histone H3 (Ser28) antibody HisH3S28-D6 at 0.1μg/mL. Cat #orb1946316.

Western blot analysis of Hela cell extract untreated or synchronized in metaphase by treatment with 0.4 ug/mL of nocodazole for 6 hr then metaphase cells were isolated by mitotic shake-off using Phospho-Histone H3 (Ser28) antibody at 1 ng/mL HisH3S28-D6. Cat. #orb1946316.

Flow cytometric analysis of Hela secondary antibody only negative control (blue) or untreated (red) or treated with nocodazole (green) using 0.05 μg/mL Phospho-Histone H3 (Ser28) antibody HisH3S28-D6. Cat. #orb1946316.

Flow cytometric analysis of HeLa cells secondary antibody only negative control (blue) or untreated (red) or treated with nocodazole (green) using Phospho-Histone H3 (Ser28) antibody HisH3S28-D6 (Cat. #orb1946316) at 0.05ug/mL or Company C antibody at 0.2ug/mL (manufacturer's recommended concentration).

Western blot analysis of HeLa cells untreated or treated with nocodazole (green) using Phospho-Histone H3 (Ser28) antibody HisH3S28-D6 (Cat. #orb1946316) at 0.005ug/mL or Company C antibody at 0.07ug/mL (manufacturer's recommended concentration) developed using the same exposure.