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Catalog Number | orb1946316 |
---|---|
Category | Antibodies |
Description | Phospho-Histone H3 (Ser28) (D6) rabbit mAb Antibody |
Clonality | Monoclonal |
Clone Number | HisH3S28-D6 |
Tested applications | FC, WB |
Reactivity | Human, Mouse |
Isotype | Rabbit IgGk |
Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Ser28 of human phospho Histone H3 |
Concentration | 0.5 mg/ml |
Dilution range | 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information. |
Conjugation | Unconjugated |
UniProt ID | Q16695 |
Storage | -20°C |
Buffer/Preservatives | 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin |
Alternative names | Histone H3.1t, H3t, H3FT, HIST3H3 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 μg/mL of isotype control Cat. #orb1946241 (orange) or untreated (red) or treated with UV and PMA (green) using Phospho-Histone H3 (Ser28) antibody HisH3S28-D6 at 0.1μg/mL. Cat #orb1946316.
Western blot analysis of Hela cell extract untreated or synchronized in metaphase by treatment with 0.4 ug/mL of nocodazole for 6 hr then metaphase cells were isolated by mitotic shake-off using Phospho-Histone H3 (Ser28) antibody at 1 ng/mL HisH3S28-D6. Cat. #orb1946316.
Flow cytometric analysis of Hela secondary antibody only negative control (blue) or untreated (red) or treated with nocodazole (green) using 0.05 μg/mL Phospho-Histone H3 (Ser28) antibody HisH3S28-D6. Cat. #orb1946316.
Flow cytometric analysis of HeLa cells secondary antibody only negative control (blue) or untreated (red) or treated with nocodazole (green) using Phospho-Histone H3 (Ser28) antibody HisH3S28-D6 (Cat. #orb1946316) at 0.05ug/mL or Company C antibody at 0.2ug/mL (manufacturer's recommended concentration).
Western blot analysis of HeLa cells untreated or treated with nocodazole (green) using Phospho-Histone H3 (Ser28) antibody HisH3S28-D6 (Cat. #orb1946316) at 0.005ug/mL or Company C antibody at 0.07ug/mL (manufacturer's recommended concentration) developed using the same exposure.
FC | |
Human, Mouse | |
Monoclonal | |
SureLight 488 |
FC | |
Human, Mouse | |
Monoclonal | |
APC |
FC | |
Human, Mouse | |
Monoclonal | |
FITC |
FC | |
Human, Mouse | |
Monoclonal | |
PE |
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