Phospho-Histone H3 (Ser10) (4B6) rabbit mAb Antibody

• Catalog Number: orb1946303
• Category: Antibodies
• Description: Phospho-Histone H3 (Ser10) (4B6) rabbit mAb Antibody
• Clonality: Monoclonal
• Clone Number: HisH3S10-4B6
• Tested applications: FC, WB
• Reactivity: Human
• Isotype: Rabbit IgGk
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser10 of human phospho histone H3
• Concentration: 0.5 mg/ml
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Conjugation: Unconjugated
• UniProt ID: Q16695
• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Alternative names: Histone H3.1t, H3t, H3FT, HIST3H3
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Jurkat cell extract untreated or treated with calyculin A using Phospho-Histone H3 (Ser10) antibody HisH3S10-4B6. Cat. # orb1946303.

Flow cytometric analysis of Hela cells secondary antibody only negative control (blue) or untreated (red) or treated with nocodazole (green) using Phospho-Histone H3 (Ser10) antibody HisH3S10-4B6. Cat. #orb1946303.

Western blot analysis of A549 cell extract untreated or treated with nocodazole using 0.01 μg/mL Phospho-Histone H3 (Ser10) antibody HisH3S10-4B6 Cat. #orb1946303 or Company C antibody at 0.03 μg/mL (manufacturer's recommended concentration) developed using the same exposure.