Phospho-Histone H3 (Ser10/Thr11) Rabbit mAb

• Catalog Number: orb1563537
• Category: Antibodies
• Description: Phospho-Histone H3 (Ser10/Thr11) Rabbit mAb
• Target: H3C1
• Clonality: Monoclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 0.3 mg/ml
• Buffer/Preservatives: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% rAlbumin.
• Purification: Affinity Purified
• Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Ser10/Thr11 of human Histone H3
• UniProt ID: P68431
• MW: Calculated MW: 15 kDa; Observed MW: 15 kDa
• Tested applications: ICC, IF, IHC-Fr, IHC-P, IP, WB
• Dilution range: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
• Antibody Type: Primary Antibody
• Clone Number: R08-6C4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: H3 histone; family 3A; H3 histone; family 3B (H3.3
• Research Area: Epigenetics & Chromatin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry analysis of Phospho-Histone H3 (Ser10/Thr11) (green) in Hela using Phospho-Histone H3 (Ser10/Thr11) antibody, and DAPI (blue).

Western blot analysis of Phospho-Histone H3 (Ser10/Thr11) in 3T3 lysates using Phospho-Histone H3 (Ser10/Thr11) antibody.

Western blot analysis of Histone H3 (Phospho-Ser10/Thr11) in Hela, A549, HL-60, C6 lysates using Histone H3 (Phospho-Ser10/Thr11) antibody.

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Histone H3 (Phospho-Ser10/Thr11) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.