Phospho-Histone H3 (Ser10) Rabbit pAb

• Catalog Number: orb1562964
• Category: Antibodies
• Description: Phospho-Histone H3 (Ser10) Rabbit pAb
• Clonality: Polyclonal
• Tested applications: ICC, IF, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Human Phospho-Histone H3(Ser10) synthetic peptide conjugated to KLH.
• Antibody Type: Primary Antibody
• Concentration: 2.46 mg/ml
• Dilution range: WB: 1/500-1/1000 IF: 1/50-1/200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Calculated MW: 15 kDa; Observed MW: 15 kDa
• Target: H3C1
• UniProt ID: P68431
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Alternative names: H3 histone; family 3A; H3 histone; family 3B (H3.3
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Phospho-Histone H3 (Ser10) in HeLa lysates, untreated or 1mM hydroxyurea treated for 5 hours, using Phospho-Histone H3 (Ser10) antibody.

Immunofluorescence analysis of Phospho-Histone H3 (Ser10) in Hela cells labeled using Phospho-Histone H3 (Ser10) antibody, and DAPI (blue).