Phospho-Histone H2A.X (Ser139) Antibody

SKU: orb653525

Description

Mouse monoclonal antibody to H2AFX

Research Area

Infectious Disease & Virology

Images & Validation

• Tested Applications: ICC, WB
• Dilution Range: WB - 1:2000, ICC - 1:400
• Reactivity: Human, Mouse

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Target: H2AX (HGNC:4739)
• Molecular Weight: 15145 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-H2A histone family antibody, anti-member X antibody, anti-H2A.X antibody, anti-H2a/x antibody, anti-H2AFX antibody, anti-H2AX antibody, anti-H2AX histone antibody, anti-H2AX_HUMAN antibody, anti-Histone H2A.X antibody, anti-Histone H2AX antibody

Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cells using Phospho-Histone H2A.X (Ser139) mouse mAb (1:400).

Western blot detection of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cell lysates using Phospho-Histone H2A.X (Ser139) mouse mAb (1:2000 diluted). Predicted band size: 15KDa.Observed band size: 15KDa.

Quick Database Links

Gene Symbol

H2AX (HGNC:4739)

UniProt

P16104