Phospho-Histone H2A.X (Ser139) (1E4) rabbit mAb

• Catalog Number: orb1946178
• Category: Antibodies
• Description: Phospho-Histone H2A.X (Ser139) (1E4) rabbit mAb
• Clonality: Monoclonal
• Isotype: Rabbit IgGk
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Purification: Protein A+G
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser139 of human phospho histone H2A.X.
• UniProt ID: P16104
• Tested applications: FC, WB
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Cross Reactivity: Predicted to work with mouse, rat, and other homologues.
• Clone Number: HisH2AXS139-1E4
• Storage: -20°C
• Alternative names: H2AFX, H2AX, γH2AX, gamma-H2AX
• Note: For research use only

Flow cytometric analysis of 3T3 cells secondary antibody only (blue) or untreated with 0.1 μg/mL of isotype control Cat. #orb1946241 (orange) or untreated (red) or UV and PMA-treated (green) using phospho-Histone H2A.X (Ser139) antibody HisH2AXS139-1E4 Cat. #orb1946178.

Peptide blocking flow cytometric analysis of 293T cells secondary antibody only negative control (blue) or untreated (red) or treated with UV and PMA (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-Histone H2A.X (Ser139) antibody HisH2AXS139-1E4 at 0.1 μg/mL. Cat. #orb1946178.

Western blot analysis of HEK293T cell extract untreated or treated with UV using 0.05 μg/mL Phospho-Histone H2A.X (Ser139) antibody HisH2AXS139-1E4. Cat. #orb1946178.

Flow cytometric analysis of 293T cells secondary antibody only negative control (blue) or untreated (red) or treated with UV and PMA (green) using Phospho-Histone H2A.X (Ser139) antibody HisH2AXS139-1E4 at 0.05 μg/mL. Cat. #orb1946178.

Flow cytometric analysis of 293T cells secondary antibody only negative control (blue) or untreated (red) or treated with UV + TPA (green) using Phospho-Histone H2A.X (Ser139) antibody HisH2AXS139-1E4 (Cat. #orb1946178) at 0.1ug/mL or Company C antibody at 0.3ug/mL (manufacturer's recommended concentration).