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Description
Research Area
Images & Validation
−| Tested Applications | IP, WB |
|---|---|
| Dilution Range | WB: 1/500-1/1000 IP: 1/20 |
| Reactivity | Hamster, Human, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone No. | R05-2H9 |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Thr185 of human ERK2 |
| Target | MAPK1/MAPK3 |
| Molecular Weight | Calculated MW: 44,42 kDa; Observed MW: 44,42 kDa |
| Purification | Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% rAlbumin. |
| Concentration | 0.3 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Western blot analysis of Phospho-ERK1 (Thr202) /ERK2 (Thr185) in Hela, Hela+TPA lysates using Phospho-ERK1/2 (Thr202/Thr185) antibody.

Western blot analysis of Phospho-p44/42 MAPK (ERK1/2) (Thr202) in C6, CHO-K1 lysates using Phospho-p44/42 MAPK (ERK1/2) (Thr202) antibody.
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Protocol Information
Phospho-ERK1/2 (Thr202/Thr185) Rabbit mAb (orb1563089)
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