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MAPK1/MAPK3 Antibody
SKU: orb1258906
Featured
Description
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−Item 1 of 7
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant Protein of human ERK1 / ERK2 |
| Target | MAPK1/MAPK3 |
| Molecular Weight | Observed: 42kDa/44kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ERK, ERT1, MAPK 1, MAPK2, ERK2
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Western blot analysis of extracts of various cell lines, using ERK1/ERK2 antibody (orb1258906) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunofluorescence analysis of C6 cells using ERK1/ERK2 antibody (orb1258906) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using ERK1/ERK2 antibody (orb1258906) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using ERK1/ERK2 antibody (orb1258906) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using ERK1/ERK2 Polyclonal Antibody (orb1258906) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using ERK1/ERK2 Polyclonal Antibody (orb1258906) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using ERK1/ERK2 Polyclonal Antibody (orb1258906) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
MAPK1/MAPK3 Antibody (orb1258906)
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