Phospho-Chk1 (Ser345) (R3F9) rabbit mAb Antibody

• Catalog Number: orb1946212
• Category: Antibodies
• Description: Phospho-Chk1 (Ser345) (R3F9) rabbit mAb Antibody
• Clonality: Monoclonal
• Clone Number: Chk1S345-R3F9
• Tested applications: FC
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgGk
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser345 of human phospho Chk1
• Concentration: 0.5 mg/ml
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Conjugation: Unconjugated
• UniProt ID: O14757
• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Alternative names: Serine/threonine-protein kinase Chk1, CHK1 checkpo
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of Hela cells secondary antibody only negative control (blue) or untreated (red) or treated with UV plus TPA (green) using Phospho-Chk1 (S345) antibody Chk1S345-R3F9 1μg/mL. Cat. #orb1946212.

Peptide blocking flow cytometric analysis of Hela cells secondary antibody only negative control (light blue) or untreated (red) or treated with calyculin A (green) or untreated and blocked with phospho-peptide (black) or CalA and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or CalA and blocked with non-phospho peptide (purple) using Phospho-Chk1 (S345) antibody Chk1S345-R3F9 0.01μg/mL. Cat. #orb1946212.

Chk1S345-R3F9 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of NIH3T3 cells secondary antibody only (blue) or 0.1 μg/mL of isotype control Cat. #orb1946241 (orange) or of Phospho-Chk1 (S345) antibody Chk1S345-R3F9 (green) Cat. #orb1946212.

Flow cytometric analysis of C6 cells secondary antibody only negative control (blue) or treated with imatinib (red) or treated with pervanadate (green) using Phospho-Chk1 (S345) antibody Chk1S345-R3F9 0.01μg/mL. Cat. #orb1946212.