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Catalog Number | orb1946155 |
---|---|
Category | Antibodies |
Description | Phospho-Chk1 (Ser317) (G1) rabbit mAb Antibody |
Clonality | Monoclonal |
Clone Number | Chk1S317-G1 |
Tested applications | FC, WB |
Reactivity | Human, Mouse |
Isotype | Rabbit IgGk |
Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Ser317 of human phospho Chk1 |
Concentration | 0.5 mg/ml |
Dilution range | 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information. |
Conjugation | Unconjugated |
UniProt ID | O14757 |
Storage | -20°C |
Buffer/Preservatives | 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin |
Alternative names | Serine/threonine-protein kinase Chk1, CHK1 checkpo Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Peptide blocking flow cytometric analysis of K562 cells secondary antibody only negative control (light blue) or using 0.1 μg/mL of isotype control Cat. #orb1946241 (orange) or treated with pervanadate and using 0.1 μg/mL of Phospho-Chk1 (S317) antibody Chk1S317-G1 Cat. #orb1946155 (green) or pervanadate and blocked with phospho peptide (black) or pervanadate and blocked with non-phospho peptide (purple).
Western blot analysis of COS7 cell extract untreated or treated with UV + TPA using Phospho Chk1 (S317) antibody ChK1S317-G1 at 0.01 μg/mL. Cat. #orb1946155.
Peptide blocking flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (light blue) or using 0.1 μg/mL of isotype control Cat. #orb1946241 (orange) or treated with IFNα + IL4 + pervanadate and using 0.1 μg/mL of Phospho-Chk1 (S317) antibody Chk1S317-G1 Cat. #orb1946155 (green) or IFNα + IL4 + Pv and blocked with phospho peptide (black) or IFNα + IL4 + Pv and blocked with non-phospho peptide (purple).
Flow cytometric analysis of K562 cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNα + IL4 + pervanadate (green) using Phospho-Chk1 (S317) antibody Chk1S317-G1 0.1 μg/mL. Cat. #orb1946155.
FC | |
Human, Mouse | |
Monoclonal | |
SureLight 488 |
FC | |
Human, Mouse | |
Monoclonal | |
APC |
FC | |
Human, Mouse | |
Monoclonal | |
FITC |
FC | |
Human, Mouse | |
Monoclonal | |
PE |
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