Phospho-4E BP1 (Thr46) Antibody

• Catalog Number: orb1733129
• Category: Antibodies
• Description: Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation. Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity.
• Species/Host: Rabbit
• Clonality: Monoclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Thr45 of human eIF4EBP1
• Concentration: 1mg/ml
• Dilution range: IP: 1/20; WB: 1/500-1/1000; IHC: 1/50-1/100
• Conjugation: Unconjugated
• MW: Calculated MW: 13 kDa; Observed MW: 15-20 kDa
• Entrez: 1978
• UniProt ID: Q13541
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Buffer/Preservatives: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin
• Alternative names: EIF4EBP1; Eukaryotic translation initiation factor
• Note: For research use only
• Expiration Date: 12 months from date of receipt.