PHLDA3 Antibody

SKU: orb1247108

Description

PHLDA3 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:In transfected HEK293 transiently expressing full-length Human PHLDA3 (myc and DYKDDDDK tagged), a band of approx. 18kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration: 0.2-1µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-RARQSLGTGTLVS
• Target: PHLDA3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PHLDA3, TIH1, pleckstrin homology-like domain, family A, member 3, pleckstrin homology-like domain, family A, member 2, OTTHUMP00000033920

HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Human PHLDA3 with C-terminal MYC tag probed with orb1247108 (1 ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with orb1247108 (1 mg/ml) in Lane B.

Quick Database Links

Gene Symbol

PHLDA3

UniProt

Q9Y5J5

RefSeq (Protein):NP_036528.1