PHIP Antibody

• Catalog Number: orb1256528
• Category: Antibodies
• Description: PHIP Antibody
• Target: PHIP
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1542-1821 of human PHIP (NP_060404.3).
• UniProt ID: Q8WWQ0
• MW: Observed: 250kDa
• Tested applications: IF, IHC, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: BRWD2, DCAF14, DIDOD, WDR11, ndrp, PH-interacting
• Note: For research use only

Western blot analysis of extracts of various cell lines, using PHIP antibody (orb1256528) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using PHIP antibody (orb1256528) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human kidney cancer using PHIP antibody (orb1256528) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse tumor using PHIP antibody (orb1256528) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of MCF-7 cells using PHIP antibody (orb1256528).