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Catalog Number | orb1527316 |
---|---|
Category | Antibodies |
Description | PHIP Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP, WB |
Reactivity | Human |
Immunogen | Between 1771 and C-terminus |
Antibody Type | Primary Antibody |
Concentration | 200 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | PHIP |
UniProt ID | Q8WWQ0 |
NCBI | NP_060404.3 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | PH-interacting protein; DCAF14; WDR11; WD repeat-c Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human PHIP by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PHIP antibody orb1527316 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. PHIP was also immunoprecipitated by rabbit anti-PHIP antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes seconds (A and B).
IF, IH, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |