PHB Antibody

SKU: orb1264666

Description

PHB Antibody

Research Area

Cell Biology, Metabolism Research

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Gallus
• Application Notes:
  For IHC-P starting dilution is: 1:10~50For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Immunogen: This PHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-117 amino acids from the Central region of human PHB.
• Target: PHB
• Molecular Weight: 30 kDa
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Prohibitin, PHB

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PHB antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Western blot analysis of PHB Antibody in Hela cell line lysates (35 ug/lane)

Western blot analysis of PHB using rabbit polyclonal PHB Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PHB gene.

Confocal immunofluorescent analysis of PHB Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Quick Database Links

Gene Symbol

PHB

UniProt

P35232

RefSeq:P35232