PHB Rabbit Polyclonal Antibody

• Catalog Number: orb574394
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PHB
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Zebrafish
• Reactivity: Human
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human PHB
• Concentration: 0.5 mg/ml
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Conjugation: Unconjugated
• MW: 30kDa
• Target: PHB
• UniProt ID: P35232
• Protein Sequence: Synthetic peptide located within the following region: AEQQKKAAIISAEGDSKAAELIANSLATAGDGLIELRKLEAAEDIAYQLS
• NCBI: NP_002625
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: PHB1, HEL-215, HEL-S-54e
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample Tissue: Human 293T, Antibody dilution: 1.0 ug/ml.

Sample Type: 293 Cell lysates, Antibody dilution: 1.0 ug/ml.

Sample Type: 721_b Cell lysates, Antibody dilution: 1.0 ug/ml.

Sample Type: Daudi Cell lysates, Antibody dilution: 1.0 ug/ml.

Human Stomach

PHB antibody - C-terminal region (orb574394), Catalog Number: orb574394, Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue, Observed Staining: Cytoplasm in Human Bronchial Epithelial Tissue, Primary Antibody Concentration: 1:600, Secondary Antibody: Donkey anti-Rabbit-Cy3, Secondary Antibody Concentration: 1:200, Magnification: 20X, Exposure Time: 0.5-2.0 sec.

PHB antibody - C-terminal region (orb574394), Catalog Number: orb574394, Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue, Observed Staining: Cytoplasm in hepatocytes, Primary Antibody Concentration: 1:600, Secondary Antibody: Donkey anti-Rabbit-Cy3, Secondary Antibody Concentration: 1:200, Magnification: 20X, Exposure Time: 0.5-2.0 sec.

PHB antibody - C-terminal region (orb574394), Catalog Number: orb574394, Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue, Observed Staining: Cytoplasm of pneumocytes, Primary Antibody Concentration: 1:600, Secondary Antibody: Donkey anti-Rabbit-Cy3, Secondary Antibody Concentration: 1:200, Magnification: 20X, Exposure Time: 0.5-2.0 sec.

WB Suggested Anti-PHB Antibody Titration: 0.2-1 ug/ml, Positive Control: Jurkat cell lysate, PHB is supported by BioGPS gene expression data to be expressed in Jurkat.