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PHB Rabbit Polyclonal Antibody
SKU: orb574394
Description
Images & Validation
−Item 1 of 9
| Tested Applications | IHC, WB |
|---|---|
| Reactivity | Human |
| Predicted Reactivity | Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Zebrafish |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Immunogen | The immunogen is a synthetic peptide directed towards the C terminal region of human PHB |
| Target | PHB |
| Molecular Weight | 30kDa |
| Purification | Affinity Purified |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Concentration | 0.5 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−PHB1, HEL-215, HEL-S-54e
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Sample Tissue: Human 293T, Antibody dilution: 1.0 ug/ml.
Sample Type: 293 Cell lysates, Antibody dilution: 1.0 ug/ml.
Sample Type: 721_b Cell lysates, Antibody dilution: 1.0 ug/ml.
Sample Type: Daudi Cell lysates, Antibody dilution: 1.0 ug/ml.
Human Stomach
PHB antibody - C-terminal region (orb574394), Catalog Number: orb574394, Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue, Observed Staining: Cytoplasm in Human Bronchial Epithelial Tissue, Primary Antibody Concentration: 1:600, Secondary Antibody: Donkey anti-Rabbit-Cy3, Secondary Antibody Concentration: 1:200, Magnification: 20X, Exposure Time: 0.5-2.0 sec.
PHB antibody - C-terminal region (orb574394), Catalog Number: orb574394, Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue, Observed Staining: Cytoplasm in hepatocytes, Primary Antibody Concentration: 1:600, Secondary Antibody: Donkey anti-Rabbit-Cy3, Secondary Antibody Concentration: 1:200, Magnification: 20X, Exposure Time: 0.5-2.0 sec.
PHB antibody - C-terminal region (orb574394), Catalog Number: orb574394, Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue, Observed Staining: Cytoplasm of pneumocytes, Primary Antibody Concentration: 1:600, Secondary Antibody: Donkey anti-Rabbit-Cy3, Secondary Antibody Concentration: 1:200, Magnification: 20X, Exposure Time: 0.5-2.0 sec.
WB Suggested Anti-PHB Antibody Titration: 0.2-1 ug/ml, Positive Control: Jurkat cell lysate, PHB is supported by BioGPS gene expression data to be expressed in Jurkat.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
PHB Rabbit Polyclonal Antibody (orb574394)
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