UchL1 Antibody

SKU: orb2645182

Description

This mAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, UchL1 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for UchL1 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in the gene is believed to cause a form of Parkinson's disease.

Research Area

Cell Biology

Images & Validation

• Tested Applications: WB
• Dilution Range: Western blot: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  The optimal dilution of the anti-UchL1 antibody for each application should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: SPM575
• Immunogen: Native UchL1/PGP9.5 protein isolated from human brain was used as the immunogen for this anti-UchL1 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot testing of human brain lysate with anti-UchL1 antibody (clone SPM575). Predicted molecular weight ~25 kDa.

SDS-PAGE analysis of purified, BSA-free anti-UchL1 antibody (clone SPM575) as confirmation of integrity and purity.

Quick Database Links

UniProt

P09936