PFKM rabbit pAb

SKU: orb766056

Description

Rabbit polyclonal antibody to PFKM

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human PFK-1. AA range:320-369
• Molecular Weight: 85
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-PFKM antibody, anti-PFKX antibody, anti-6-phosphofructokinase antibody, anti- muscle type antibody, anti-Phosphofructo-1-kinase isozyme A antibody, anti-PFK-A antibody, anti-Phosphofructokinase-M antibody, anti-Phosphofructokinase 1 antibody, anti-Phosphohexokinase antibody

Western Blot analysis of various cells using PFKM Polyclonal Antibody.

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive control (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemistry analysis of PFK-1 antibody in paraffin-embedded human brain tissue.

Western blot analysis of lysate from Hela cells, using PFK-1 antibody.