Fructose 6 Phosphate Kinase (PFKM) Antibody (C-term)

• Catalog Number: orb1928573
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB3908
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:10~50, WB: 1:1000, IHC-P: 1:50~100, IHC-P: 1:50~100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 85183 Da
• Target: This Fructose 6 Phosphate Kinase (PFKM) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 746-776 amino acids from the C-terminal region of human Fructose 6 Phosphate Kinase (PFKM).
• UniProt ID: P08237
• NCBI: NP_001160160.1, NP_001160159.1, NP_001160158.1, NP_000280.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: ATP-dependent 6-phosphofructokinase, muscle type {
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PFKM polyclonal antibody in Hela cell line lysate. PFKM (arrow) was detected using the purified Pab.

Confocal immunofluorescent analysis of Fructose 6 Phosphate Kinase (PFKM) Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PFKM antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PFKM Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.