Perifosine

SKU: orb1305401

Description

Perifosine

Research Area

Cell Biology, Signal Transduction

Key Properties

• CAS Number: 157716-52-4
• MW: 461.66
• Purity: 99.94% (May vary between batches)
• Formula: C₂₅H₅₂NO₄P
• SMILES: O(P(OCCCCCCCCCCCCCCCCCC)(=O)[O-])C1CC[N+](C)(C)CC1
• Target: Apoptosis|||Akt|||Autophagy
• Solubility: H2O:10 mM;DMSO:Insoluble

Bioactivity

• Target IC50:
  Akt:4.7 μM
• In Vivo:
  In MM.1S cells, Perifosine (10 μM) completely inhibited Akt phosphorylation. In immortalized keratinocytes and head and neck squamous carcinoma cells (IC50=0.6-8.9 μM), Perifosine inhibited cell proliferation. In mouse glial progenitor cells, Perifosine induced cell cycle arrest at G1 and G2 by reducing the phosphorylation level of Akt and extracellular signal-regulated kinases, causing cell growth inhibition. In human hepatocellular carcinoma cells, Perifosine induced cell cycle arrest and apoptosis by blocking Akt phosphorylation.
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• In Vitro:
  In MM.1S cells, Perifosine (10 μM) completely inhibited Akt phosphorylation. In immortalized keratinocytes and head and neck squamous carcinoma cells (IC50=0.6-8.9 μM), Perifosine inhibited cell proliferation. In mouse glial progenitor cells, Perifosine induced cell cycle arrest at G1 and G2 by reducing the phosphorylation level of Akt and extracellular signal-regulated kinases, causing cell growth inhibition. In human hepatocellular carcinoma cells, Perifosine induced cell cycle arrest and apoptosis by blocking Akt phosphorylation.
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• Cell Research:
  Cells are incubated in the medium with 10% FCS for 48 hours with indicated concentration of Periosine. Cell viability is determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. (Cell Proliferation Kit I; Roche). The absorbance at 590 nm is recorded using the 96-well plate reader.(Only for Reference)
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Protein kinase B, PKB, Perifosine, Akt, Autophagy, Apoptosis, D 21266, D21266, D-21266, Inhibitor, KRX 0401, KRX0401, KRX-0401, NSC 639966, inhibit, NSC-639966, NSC639966

Compound Identifiers

PubChem CID

148177