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Description
Research Area
Immunology & Inflammation
Images & Validation
−
| Tested Applications | IHC, WB |
|---|---|
| Dilution Range | Western Blot (1-2ug/ml);Immunohistochemistry (Formalin-fixed) (1:50-1:100 for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | 1A10 |
| Immunogen | Prokaryotic recombinant fusion protein corresponding to a portion of the extracellular domain downstream of the signal sequence of the CD31 (PECAM-1) |
| Target | PECAM-1 |
| Molecular Weight | ~100kDa (endothelium) and ~130kDa (platelets)' |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Tissue culture supernatant with 0.05% Azide. Contact us if you require it in a different format. |
| Concentration | Culture Supernatant |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−EndoCAM; PECA1; Platelet Endothelial Cell Adhesion Molecule 1; GPIIA'
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry































































