PECAM1 Antibody

SKU: orb1252720

Description

PECAM1 Antibody

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Reactivity: Human, Primate, Rabbit
• Application Notes:
  Flow Cytometry: 0.5-1ug/10e6 cellsIF: 1-2 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the PECAM-1 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: C31.7
• Immunogen: Human recombinant PECAM-1 / CD31 protein was used as the immunogen for this antibody.
• Target: PECAM1
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Disclaimer: For research use only

Alternative Names

Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, CD31, PECAM1

IHC staining of colon carcinoma with PECAM-1 antibody (C31.7). Note cell membrane staining of endothelial cells.

IHC staining of angiosarcoma with PECAM-1 antibody (C31.7).

IHC staining of tonsil tissue with PECAM-1 antibody (C31.7).

Western blot testing of human samples using PECAM-1 antibody (C31.7).

FACS testing of Jurkat cells: Black = cells alone; Green = isotype control; Red = PECAM-1 antibody PE conjugate.

SDS-PAGE Analysis of Purified, BSA-Free PECAM-1 Antibody (clone C31.7). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

Gene Symbol

PECAM1

UniProt

P16284