PDGF beta (PDGFB) Antibody

• Catalog Number: orb751251
• Category: Antibodies
• Description: Mouse monoclonal antibody to PDGF B
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PDGFB/3072
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG2c
• Immunogen: Recombinant fragment of human PDGFB protein (around aa 27-158) (exact sequence is proprietary)
• Concentration: 200 μg/ml
• Dilution range: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 14kDa (monomeric B chain); 31-35kDa (dimer)
• Target: PDGFB
• Entrez: 5155
• UniProt ID: P01127
• Expression System: Bioreactor
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Alternative names: Anti-Becaplermin antibody, anti-c sis antibody, an
• Note: For research use only
• Application notes: Positive Control: Human colon, kidney or lymph node.
• Expiration Date: 12 months from date of receipt.