Pdcd4 (phospho-S457) antibody

• Catalog Number: orb345560
• Category: Antibodies
• Description: Pdcd4 (phospho-S457) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Anti-Pdcd4 pS457 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding Ser457 in the human Pdcd4 protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:400,000, IHC: 1.25 - 2.5 µg/ml, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Pdcd4 pS457 was affinity purified from monospecific antiserum by immunoaffinity chromatography using phospho-peptide coupled to agarose beads followed by solid phase adsorption against nonphospho-peptide. This antibody is specific for human Pdcd4 protein phosphorylated at Ser457. A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with Pdcd4 from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: Q53EL6
• NCBI: 21735596
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-PDCD4 pS457 antibody, PDCD-4, PDCD 4,
• Note: For research use only
• Application notes: Anti-Pdcd4 pS457 affinity purified antibody has been tested for use in ELISA, immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. By western blot, a band approximately 52 kDa in size corresponding to Pdcd4 protein is expected in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Lanes 1 & 2 each contain 100 ng recombinant Pdcd4. Lanes 3 & 4 each contain 30 ug of whole cell extract from 293 HEK cells using Pdcd4 (phospho-S457) antibody

Immunohistochemical staining of multi-human, multi-brain and multi-cancer slides using Pdcd4 (phospho-S457) antibody