Pdcd4 (phospho-S457) antibody

• Catalog Number: orb345559
• Category: Antibodies
• Description: Pdcd4 (phospho-S457) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding Ser457 in the human Pdcd4 protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:400,000, IHC: 21.25 - 2.5 µg/ml, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phospho-peptide coupled to agarose beads followed by solid phase adsorption against nonphospho-peptide. This antibody is specific for human Pdcd4 protein phosphorylated at Ser457. A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with Pdcd4 from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: Q53EL6
• NCBI: 21735596
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-PDCD4 pS457 antibody, PDCD-4, PDCD 4,
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. By western blot, a band approximately 52 kDa in size corresponding to Pdcd4 protein is expected in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Lanes 1 & 2 each contain 100 ng recombinant Pdcd4. Lanes 3 & 4 each contain 30 ug of whole cell extract from 293 HEK cells using Pdcd4 (phospho-S457) antibody

Immunohistochemical staining of variety of tissues including multi-human, multi-brain and multi-cancer slides using Pdcd4 (phospho-S457) antibody