Recombinant PD-L1 Antibody / B7-H1 / CD274

SKU: orb2643760

Description

PD-L1 is a checkpoint regulator in immune cells, it is expressed on immune or non-hematopoietic cells. Expression of the protein is seen during pregnancy where it has a role in suppressing the immune system. PD-L1 induces an inhibitory signal in activated T-cells and promotes T-cell apoptosis. It is overexpressed in a number of different cancers where it is believed to play a significant role in the cancerâ€s ability to evade the immune system.

Images & Validation

• Tested Applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the recombinant PD-L1 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: PDL1/4451R
• Immunogen: A portion of amino acids 39-191 from the human protein was used as the immunogen for this recombinant PD-L1 antibody.
• Purification: Protein A affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC testing of FFPE lung SCC with recombinant PD-L1 antibody (clone PDL1/4451R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE lung SCC with recombinant PD-L1 antibody (clone PDL1/4451R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Quick Database Links

UniProt

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