PD-L1 Antibody / B7-H1 / CD274

• Catalog Number: orb639912
• Category: Antibodies
• Description: PD-L1 is a checkpoint regulator in immune cells, it is expressed on immune or non-hematopoietic cells. Expression of the protein is seen during pregnancy where it has a role in suppressing the immune system. PD-L1 induces an inhibitory signal in activated T-cells and promotes T-cell apoptosis. It is overexpressed in a number of different cancers where it is believed to play a significant role in the cancerâ€s ability to evade the immune system.
• Clonality: Recombinant
• Species/Host: Mouse
• Isotype: Rabbit IgG, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A portion of amino acids 39-191 from the human protein was used as the immunogen for this recombinant PD-L1 antibody.
• UniProt ID: Q9NZQ7
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: PDL1/4451R
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Epigenetics, Immunology
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE lung SCC with recombinant PD-L1 antibody (clone PDL1/4451R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE lung SCC with recombinant PD-L1 antibody (clone PDL1/4451R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.