PCNA Rabbit Polyclonal Antibody

SKU: orb1742620

Description

PCNA antibody recognizes the proliferating cell nuclear antigen (PCNA), a cofactor of DNA polymerase delta with a predicted molecular weight of 29 kDa. PCNA is important for DNA synthesis, repair, and epigenetic modifications, and also serves as an organelle marker of the nucleus. PCNA antibody can be used for distinguishing between early, middle, and late S-phase of the cell cycle as well as grading of different neoplasms.

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: ICC, IHC-P, IP, WB
• Dilution Range: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
• Reactivity: Drosophila, Golden Hamster, Human, Mouse, Rat
• Application Notes:
  Positive control: NIH-3T3 , PC-12

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the center region of human PCNA. The exact sequence is proprietary.
• Target: proliferating cell nuclear antigen
• Molecular Weight: 29
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS, 1% rAlbumin, 20% Glycerol, 0.025% ProClin 300.
• Concentration: 0.11 mg/ml (Please refer to the vial label for the specific concentration.)
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

proliferating cell nuclear antigen , ATLD2

Quick Database Links

Gene Symbol

proliferating cell nuclear antigen

UniProt

P12004