PCNA Antibody

• Catalog Number: orb214367
• Category: Antibodies
• Description: The PCNA Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PCNA. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Bovine, Canine, Gallus, Human, Monkey, Mouse, Porcine, Rabbit, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PCNA. The exact sequence is proprietary.
• UniProt ID: P17918, P12004, P04961
• Tested applications: IF, WB
• Dilution range: WB: 1-500-1-1000, IF/ICC: 1-100-1-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Proliferating cell nuclear antigen; PCNA; Cyclin
• Research Area: Cancer, DNA Damage, Epigenetics, Infectious Diseas
• Note: For research use only
• Entrez: 18538, 25737, 5111

References

Zhou, Xiaohu et al. Metformin Inhibit Lung Cancer Cell Growth and Invasion in Vitro as Well as Tumor Formation in Vivo Partially by Activating PP2A Med. Sci. Monit., 25, 836-846 (2019)

PubMed: 30693913

Western blot analysis of PCNA expression in HEK293T (A), mouse testis (B), rat testis (C) whole cell lysates. (Predicted band size: 28 kD; Observed band size: 36 kD)

Immunofluorescent analysis of PCNA staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.