PAKα rabbit pAb

• Catalog Number: orb766021
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PAK1
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human PAK1. AA range:178-227
• MW: 60
• Tested applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-PAK1 antibody, anti-Serine/threonine-protein
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of various cells using PAKα Polyclonal Antibody diluted at 1:500.

Immunofluorescence analysis of HeLa cells, using PAK1 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using PAK1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 293 cells, treated with Etoposide 25uM 60', using PAK1 Antibody. The lane on the right is blocked with the synthesized peptide.