PAK5 Antibody

SKU: orb1928757

Description

PAK5 Antibody

Research Area

Cell Biology, Immunology & Inflammation, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution range: IHC-P-Leica - 1:500, WB - 1:2000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 80745 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Alternative Names

KIAA1264, PAK7

Anti-PAK5 Antibody at 1:2000 dilution + Human brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 81 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-PAK5 Antibody at 1:1000 dilution. Lane 1: SH-SY5Y whole cell lysate. Lane 2: Raji whole cell lysate. Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 81 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of anti-PAK5 Pab in lysates from transiently transfected COS7 cells. Lane 1: negative control, Lane 2: PAK1-expressing cells, Lane 3: PAK2-expressing cells, Lane 4: PAK4-expressing cells, Lane 5: PAK5-expressing cells, and Lane 6: PAK6-expressing cells. PAK5 (arrow) was detected using purified Pab.

Immunohistochemical analysis on paraffin-embedded Human cerebellum tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Quick Database Links

UniProt

Q9P286