PAK5 Antibody

• Catalog Number: orb1928757
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB1235
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, WB: 1:2000, IHC-P: 1:100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 80745 Da
• Target: This PAK5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-198 amino acids from human PAK5.
• UniProt ID: Q9P286
• NCBI: NP_065074.1, NP_817127.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Serine/threonine-protein kinase PAK 7, p21-activat
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-PAK5 Antibody at 1:2000 dilution + Human brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 81 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-PAK5 Antibody at 1:1000 dilution. Lane 1: SH-SY5Y whole cell lysate. Lane 2: Raji whole cell lysate. Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 81 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of anti-PAK5 Pab in lysates from transiently transfected COS7 cells. Lane 1: negative control, Lane 2: PAK1-expressing cells, Lane 3: PAK2-expressing cells, Lane 4: PAK4-expressing cells, Lane 5: PAK5-expressing cells, and Lane 6: PAK6-expressing cells. PAK5 (arrow) was detected using purified Pab.

Immunohistochemical analysis on paraffin-embedded Human cerebellum tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.