PAH Antibody (Center)

• Catalog Number: orb1927180
• Category: Antibodies
• Description: Purified Mouse Monoclonal Antibody (Mab)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 717CT21.1.1
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1,κ
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, WB: 1:1000, WB: 1:1000, IHC-P: 1:25, IHC-P: 1:25, IHC-P: 1:25
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 51862 Da
• Target: This PAH antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 127-161 amino acids from the Central region of human PAH.
• UniProt ID: P00439
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Phenylalanine-4-hydroxylase, PAH, Phe-4-monooxygen
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin-embedded H. liver section using PAH Antibody (Center). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded M. kidney section using PAH Antibody (Center). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

All lanes: Anti-PAH Antibody (Center) at 1:1000 dilution. Lane 1: C6 whole cell lysate. Lane 2: Rat brain lysate. Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/8000 dilution.Observed band size: 52 KDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-PAH Antibody (Center) at 1:1000 dilution. Lane 1: C6 whole cell lysate. Lane 2: Rat brain lysate. Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/8000 dilution.Observed band size: 52 KDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-PAH Antibody (Center) at 1:1000 dilution. Lane 1: C6 whole cell lysate. Lane 2: Rat brain lysate. Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/8000 dilution.Observed band size: 52 KDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of lysates from HepG2 cell line and mouse liver rat liver tissue lysates (from left to right) using PAH Antibody (Center). Diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1: 3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.

Staining PAH in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.