PAH Antibody

• Catalog Number: orb1474131
• Category: Antibodies
• Description: The PAH Antibody is suitable for IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PAH. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column.
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: This antibody is purified through a protein G column.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PAH. The exact sequence is proprietary.
• UniProt ID: P04176, P00439, P16331
• Tested applications: IHC, WB
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/200)
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Phenylalanine-4-hydroxylase; PAH; Phe-4-monooxygen
• Note: For research use only
• Entrez: 18478, 5053
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PAH expression in HepG2 (A), mouse liver (B), rat liver (C) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 52 kD)

Immunohistochemical analysis of PAH staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.