PAFAH1B1 Antibody

SKU: orb1247928

Description

PAFAH1B1 Antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 40kDa band observed in Human Ovary and in Rat Ovary lysates (calculated MW of 46.6kDa according to Human NP_000421.1 and to Rat NP_113951.1). Recommended concentration: 0.1-0.3µg/mL.Immunohistochemistry:Paraffin embedded Human Brain (Cortex), Thyroid and Pancreas. Recommended concentration: 3.75µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: TGSVDQTVKVWECR
• Target: PAFAH1B1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PAFAH1B1, LIS1, platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45kDa, MDCR, PAFAH, lissencephaly 1 protein, Platelet-activating factor acetylhydrolase, isoform 1B, alpha subunit, platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit (45kD), platelet-activating factor acetylhydrolase, isoform Ib, subunit 1 (45kDa), LIS2, MDS,

orb1247928 (3.75 ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.

orb1247928 (3.75 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247928 (0.1 ug/ml) staining of Rat Ovary lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247928 (3.75 ug/ml) staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.

Quick Database Links

Gene Symbol

PAFAH1B1

UniProt

P43034

RefSeq (Protein):NP_000421.1