PAD4 Antibody

• Catalog Number: orb1247150
• Category: Antibodies
• Description: PAD4 Antibody
• Target: PADI4
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: AQGTLIRVTPEQPC
• UniProt ID: Q9UM07
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 74kDa band observed in Human Spleen lysates (calculated MW of 74.1kDa according to NP_036519.2). In transfected HEK293 transiently expressing PADI4 a band of approx. 75kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 5ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PADI4, PAD, PDI4, PDI5, PADI5, peptidyl arginine d
• Note: For research use only
• NCBI: NP_036519.2

orb1247150 (0.3 ug/ml) staining of Human Spleen lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

HEK293 overexpressing PADI4 and probed with orb1247150 (mock transfection in first lane).

orb1247150 (5 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.