Goat anti-PAD4 / PADI4 Antibody

• Catalog Number: orb18935
• Category: Antibodies
• Description: Goat polyclonal antibody to PADI4
• Target: PAD4 / PADI4
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: AQGTLIRVTPEQPC
• RRID: AB_10755747
• MW: 74.1
• Tested applications: ELISA, IF, IHC
• Dilution range: ELISA: 1:64000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 74kDa band observed in Human Spleen and Liver lysates (calculated MW of 74.1kDa according to NP_036519.2). In transfected HEK293 transiently expressing PADI4 a band of approx. 75kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 μg/ml..
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti PADI4 antibody, anti PAD antibody, anti PDI4
• Research Area: Epigenetics, Immunology
• Note: For research use only
• Entrez: 23569

HEK293 overexpressing PADI4 (RC206501) and probed with orb18935 (mock transfection in first lane), tested by Origene.

Western blot analysis of Human Spleen lysate using PADI4 antibody

Immunohistochemical staining of Human Spleen using PADI4 antibody