Goat anti-PAD4 / PADI4 Antibody

• Catalog Number: orb18935
• Category: Antibodies
• Description: Goat polyclonal antibody to PADI4
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC
• Reactivity: Human
• Dilution range: ELISA: 1:64000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml
• Conjugation: Unconjugated
• MW: 74.1
• Target: PAD4 / PADI4
• Entrez: 23569
• Protein Sequence: AQGTLIRVTPEQPC
• RRID: AB_10755747
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti PADI4 antibody, anti PAD antibody, anti PDI4
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 74kDa band observed in Human Spleen and Liver lysates (calculated MW of 74.1kDa according to NP_036519.2). In transfected HEK293 transiently expressing PADI4 a band of approx. 75kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 μg/ml..
• Expiration Date: 12 months from date of receipt.

HEK293 overexpressing PADI4 (RC206501) and probed with orb18935 (mock transfection in first lane), tested by Origene.

Western blot analysis of Human Spleen lysate using PADI4 antibody

Immunohistochemical staining of Human Spleen using PADI4 antibody