TP53 Antibody

SKU: orb420365

Description

p53 K305Ac antibody

Images & Validation

• Tested Applications: DOT, ELISA, WB
• Dilution Range: ELISA: 1:5000 - 1:10000, WB: 1:1000
• Reactivity: Human
• Application Notes:
  p53 K305 Ac Antibody has been tested by dot blot and western blotting and is suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~43.6 kDa in size corresponding to p53 by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-p53 K305 Ac antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysine 305 of human p53.
• Target: TP53
• Purity: Anti-p53 K305 Ac antibody is directed against human p53 protein. p53 K305 Ac Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis used to suggest reactivity with this protein from human based on 100% homology for the immunizing sequence.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-p53 Ac-Lys305 antibody, rabbit anti-p53 K305ac antibody, cellular tumor antigen p53, tumor suppressor p53, phosphoprotein p53, Antigen NY-CO-13, TP53

Dot Blot of Rabbit Anti-p53 305KAc Antibody. Lane 1: Unmodified p53 peptide. Lane 2: 305KAc p53 peptide. Loaded: 100, 10, 1 picomoles of peptide. Primary Antibody: Left Membrane: 1.0 µg/ml of purified antibody; Middle Membrane: 0.5 µg/ml; Right Membrane: 0.25 µg/ml. Blocking: BLOTTO Blocking Buffer (p/n orb348624) for 30 min at RT. Secondary Antibody: Gt-A-Rb IgG HRP (p/n orb347654) at 1:40000.

Western Blot of RABBIT Anti-p53 (ac Lys305) Antibody. Lane 1: Ladder Opal PreStained. Lane 2: MCF-7 (p/n orb348664). Lane 3: MCF-7 Nuclear Extract (p/n orb348687). Lane 4: Jurkat Lysate (p/n orb348674). Lane 5: HeLa Whole Cell Lysate (p/n orb348668). Lane 6: A431 Whole Cell Lysate (p/n orb348665). Loaded at 10 ug. Blocking: BlockOut Buffer (p/n orb348644) for 30 min at RT. Primary Antibody: Rb anti-p53 K305 Ac at 1 ug/ml for 4°C overnight. Secondary Antibody: Gt-a-Rb HRP (p/n orb347654) at 1:70000 in orb348644 for 30 min at RT. Expected: ~53 kDa.

Quick Database Links

Gene Symbol

TP53

UniProt

P04637

RefSeq (Protein):NP_000537.3