TP53 Antibody

SKU: orb420357

Description

p53 K292 Ac antibody

Images & Validation

• Tested Applications: DOT, WB
• Dilution Range: WB: 1:1000
• Reactivity: Human
• Application Notes:
  p53 K292 Ac Antibody has been tested by dot blot and western blotting and is suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~43.6 kDa in size corresponding to p53 by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-p53 K292 Ac antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysine 292 of human p53.
• Target: TP53
• Purity: Anti-p53 K292 Ac antibody is directed against human p53 protein. p53 K292 Ac Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis used to suggest reactivity with this protein from human based on 100% homology for the immunizing sequence.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-p53 Ac-Lys292 antibody, rabbit anti-p53 K292ac antibody, cellular tumor antigen p53, tumor suppressor p53, phosphoprotein p53, Antigen NY-CO-13, TP53

Dot Blot of Rabbit Anti-p53 [ac Lys292] Antibody. Lane 1: Unmodified peptide. Lane 2: p53 k292-ac peptide. Load: 100, 10 and 1 picomoles of peptide. Primary antibody: Histone p53 K292-ac antibody at 1:1000 and 1:500 for 1 hr at 4°C. Secondary antibody: Dylight™488 rabbit secondary antibody at 1:10000 for 30 min at RT. Block: 5% BLOTTO (p/n orb348624) overnight at 4°C.

Western Blot of Anti-p53 (ac Lys292) (RABBIT) Antibody. Lane 1: HCT-116 Whole Cell Lysate (p/n orb348712). Lane 2: A549 WCL (p/n orb348675). Lane 3: C5B46 Brain Lysate. Lane 4: MCF7 Nuclear Extract Lysate (p/n orb348664). Lane 5: A431 Nuclear Extract Lysate (p/n orb348665). Lane 6: HeLa WCL (p/n orb348668). Lane 7: HeLa Nuclear Extract Lysate (p/n orb348671). Lane 8: Normal Ms Brain Lysate. Lane 9: Molecular Weight Ladder PreStained. Loaded at 10 ug. Primary Antibody: Anti-p53 292kAc at 1 µg/ml overnight at 4°C. Secondary Antibody: Goat anti-Rabbit HRP (p/n orb347654) at 1:70000 for 30 min at RT. Blocking buffer: BlockOut Universal Buffer (p/n orb348644). Predicted: ~43.6 kDa.

Quick Database Links

Gene Symbol

TP53

UniProt

P04637

RefSeq (Protein):NP_000537.3